It is a truism in science that experiments raise more questions than they answer. Darwin encountered this phenomena during his experimental career as well. Case in point, in The effects of cross and self fertilisation in the vegetable kingdom Darwin relates how he started the experiments for the book:
I was at last led to make the experiments recorded in the present volume from the following circumstance. For the sake of determining certain points with respect to inheritance, and without any thought of the effects of close interbreeding, I raised close together two large beds of self-fertilised and crossed seedlings from the same plant of Linaria vulgaris. To my surprise, the crossed plants when fully grown were plainly taller and more vigorous than the self-fertilised ones. Bees incessantly visit the flowers of this Linaria and carry pollen from one to the other; and if insects are excluded, the flowers produce extremely few seeds; so that the wild plants from which my seedlings were raised must have been intercrossed during all previous generations. It seemed therefore quite incredible that the difference between the two beds of seedlings could have been due to a single act of self-fertilisation; and I attributed the result to the self-fertilised seeds not having been well ripened, improbable as it was that all should have been in this state, or to some other accidental and inexplicable cause. During the next year, I raised for the same purpose as before two large beds close together of self-fertilised and crossed seedlings from the carnation, Dianthus caryophyllus. This plant, like the Linaria, is almost sterile if insects are excluded; and we may draw the same inference as before, namely, that the parent-plants must have been intercrossed during every or almost every previous generation. Nevertheless, the self-fertilised seedlings were plainly inferior in height and vigour to the crossed.
My attention was now thoroughly aroused, for I could hardly doubt that the difference between the two beds was due to the one set being the offspring of crossed, and the other of self-fertilised flowers.
Darwin designed this first experiment to test height and fertility of crossed and self-fertilized plants of the species Ipomoea purpurea .
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